In Vitro Study of Fluorescence Detection for Protoporphyrin IX Induced from 5-Aminolevulinic Acid in Cancerous and Normal Cells
2006
Kim, M.H. (Keimyung University, Daegu, Republic of Korea) | Kim, H.J. (The Center for Traditionl Microorganism Resources, Keimyung University, Daegu, Republic of Korea) | Lee, I.S. (The Center for Traditionl Microorganism Resources, Keimyung University, Daegu, Republic of Korea) | Kim, K.C. (Keimyung University, Daegu, Republic of Korea) | Lee, C.S. (Keimyung University, Daegu, Republic of Korea), E-mail: [email protected]
To clarify the usefulness of fluorescent diagnosis for cancer, We investigated the optimal method of administrating 5-aminolevulinic acid (5-ALA) by analyzing fluorescence signal of Protoporphyrin IX (PpIX) in the cultured normal and cancer cells. 5-ALA was injected as a photosensitizer to the cervico-uterine cancer cell line (HeLa) and in normal liver cells (Chang). Chang and HeLa cells were incubated with various concentrations of 5-ALA (0-800 μg/ml). The accumulation of PplX induced by 5-ALA was in HeLa and Chang cells.
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