Comparison between SAGE and cDNA Microarray for Quantitative Accuracy in Transcript Profiling Analyses
2006
Eom, E.M. (Ewha Womans University, Seoul, Republic of Korea) | Lee, J.Y. (Ewha Womans University, Seoul, Republic of Korea) | Park, H.S. (Ewha Womans University, Seoul, Republic of Korea) | Byun, Y.J. (Ewha Womans University, Seoul, Republic of Korea) | Ha-Lee, Y.M. (Yonsei University, Wonju, Republic of Korea) | Lee, D.H. (Ewha Womans University, Seoul, Republic of Korea), E-mail: lee@ewha.ac.kr
Array-based hybridization and the serial analysis of gene expression (SAGE) are the most common approaches for high-throughput transcript analysis. Each has advantages and disadvantages. The cDNA array allows rapid screening of a large number of samples but cannot detect unknown genes. In contrast, SAGE can detect those unknown genes or transcripts but is restricted to fewer samples. Combining these two methods could provide better high-throughput analysis that allows rapid screening of both previously known and unknown genes.
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