Cooperation between Human DAF and CD59 in Protecting Cells from Human Complement-mediated Lysis
2006
Xu, Li (Wuhan University, Wuhan, Hubei, P. R. China) | Wu, Wenlan (Wuhan University, Wuhan, Hubei, P. R. China) | Zhao, Zhouzhou (Wuhan University, Wuhan, Hubei, P. R. China) | Shao, Huanjie (Wuhan University, Wuhan, Hubei, P. R. China) | Liu, Wanhong (Wuhan University, Wuhan, Hubei, P. R. China) | Liu, Hui (Wuhan University, Wuhan, Hubei, P. R. China) | Li, Wenxin (Wuhan University, Wuhan, Hubei, P. R. China), E-mail: liwxlab@whu.edu.cn
The complement (C) regulatory proteins decay accelerating factor (DAF, CD55) and CD59 could protect host cells using different mechanisms from C-mediated damage at two distinct levels within the C pathway. Co-expression of DAF and CD59 would be an effective strategy to help overcome host C-induced xenograft hyperacute rejection. In this study, we made a construct of recombinant expression vector containing DAF and CD59 cDNA and the stable cell lines were obtained by G418 selection. Extraneous genes integration and co-expression were identified by PCR, RT-PCR and Western blot analysis.
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