Search for Novel Stress-responsive Protein Components Using a Yeast Mutant Lacking Two Cytosolic Hsp70 Genes, SSA1 and SSA2
2006
Matsumoto, Rena (Chiba University, Chiba, Japan), E-mail: matsumoto-rn@aist.go.jp | Rakwal, Randeep (Human Stress Signal Research Center (HSS), AIST, Ibaraki, Japan) | Agrawal, Ganesh Kumar (Research Laboratory for Agricultural Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal) | Jung, Y.H. (Sejong University, Seoul, Republic of Korea) | Jwa, N.S. (Sejong University, Seoul, Republic of Korea) | Yonekura, Masami (Ibaraki University, Ibaraki, Japan) | Iwahashi, Hitoshi (Human Stress Signal Research Center (HSS), AIST, Ibaraki, Japan) | Akama, Kuniko (Chiba University, Chiba, Japan)
Heat shock proteins (Hsp) 70 are a ubiquitous family of molecular chaperones involved in many cellular processes. A yeast strain, ssa1/2, with two functionally redundant cytosolic Hsp70s (SSA1 and SSA2) deleted shows thermotolerance comparable to mildly heat-shocked wild type yeast, as well as increased protein synthesis and ubiquitin-proteasome protein degradation. Since mRNA abundance does not always correlate well with protein expression levels it is essential to study proteins directly. We used a gel-based approach to identify stress-responsive proteins in the ssa1/2 mutant and identified 43 differentially expressed spots.
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