Determination of Neurotoxin Gene Expression in Clostridium botulinum Type A by Quantitative RT-PCR
2006
Shin, N.R. (Korea National Institute of Health, Seoul, Republic of Korea) | Shin, J.H. (Korea National Institute of Health, Seoul, Republic of Korea) | Chun, J.H. (Korea National Institute of Health, Seoul, Republic of Korea) | Yoon, S.Y. (Korea National Institute of Health, Seoul, Republic of Korea) | Kim, B.S. (Korea National Institute of Health, Seoul, Republic of Korea) | Oh, H.B. (Korea National Institute of Health, Seoul, Republic of Korea) | Rhie, G.E. (Korea National Institute of Health, Seoul, Republic of Korea), E-mail: gerhie@nih.go.kr
Real time reverse transcription (RT)-PCR was used to quantify the expression of the botulinum neurotoxin type A (BoNT/A) gene (cntA) by normalization with the expression of 16S rRNA. The method were confirmed by monitoring the mRNA levels of cntA during growth in five type A strains. In all but one of the strains the expression of cntA mRNA was maximal in the late exponential phase, and approximately 35-fold greater than in the early exponential phase. The concentration of the extracellular BoNT/A complex detected by ELISA was highest in stationary phase.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Korea Agricultural Science Digital Library
