Functional Analysis of sprD Gene Encoding Streptomyces griseus Protease D (SGPD) in Streptomyces griseus
2006
Choi, S.S. (Myongji University, Yongin, Republic of Korea) | Kim, J.H. (Myongji University, Yongin, Republic of Korea) | Kim, J.H. (Myongji University, Yongin, Republic of Korea) | Kang, D.K. (Easy Bio System Inc., Chonan, Republic of Korea) | Kang, S.S. (Chungbuk National University, Cheongju, Republic of Korea) | Hong, S.K. (Myongji University, Yongin, Republic of Korea), E-mail: skhong@mju.ac.kr
The chromosomal sprD gene encoding Streptomyces griseus protease D (SGPD), a chymotrypsin-like protease, was disrupted in Streptomyces griseus by insertion of the neomysin-resistance gene. The production of chymotrypsin activity of sprD disruptant was not completely abolished, but delayed by 24 h, compared with that of wild-type strain. The aerial mycelial formation of sprD disruptant was retarded, and specifically the formation of spores was not observed in the central region of colonies. However, normal morphological development into spores was observed in the marginal region of colonies.
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