Identification of tobacco Burley species specific marker in several tobacco species by AFLP
2006
Lee, Y.G. (KTnG Central Research Institute, Daejeon, Republic of Korea) | Jung, S.H. (KTnG Central Research Institute, Daejeon, Republic of Korea) | Keum, W.S. (KTnG Central Research Institute, Daejeon, Republic of Korea) | Lee, J.H. (KTnG Central Research Institute, Daejeon, Republic of Korea) | Lee, C.H. (KTnG Central Research Institute, Daejeon, Republic of Korea) | Rhee, M.S. (KTnG Central Research Institute, Daejeon, Republic of Korea)
AFLP(Amplified Fragment Length Polymorphism) analysis was conducted to cultivars of tobacco, Nicotiana tabacum in order to select the cultivar-specific markers. AFLP results using 12 primer sets revealed genetic diversity among 12 field grown tobacco cultivars. Polymorphic fragments amplified by PCR was purified and cloned to identify their nucleotide sequences. From the sequences of them, 40 primer sets were designed to select cultivar-specific markers. When genomic DNA isolated from tobacco were used as PCR template, a set of primers, BrSF/BrSR showed Burley-specific band patterns.
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