In vitro Regeneration of Phragmites australis through Embryogenic Cultures
2006
Lee, J.S. (Ajou University, Suwon, Republic of Korea) | Kim, C.K. (Seoul National University, Suwon, Republic of Korea) | Kim, I.S. (Seoul National University, Seoul, Republic of Korea) | Lee, E.J. (Seoul National University, Seoul, Republic of Korea) | Choi, H.K. (Ajou University, Suwon, Republic of Korea), E-mail: [email protected]
Phragmites australis (reed) has received much attention as being one of the principle emergent aquatic plants for treating industrial and civil wastewater. Plant regeneration via plant tissue culture in P. australis was investigated. Three types of callus were identified from seeds on N6 medium plus 4.5 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Yellow compact type showed the best redifferentiation, whereas white compact type and yellow friable were not competent to differentiate into plants. Solid medium culture was better than liquid suspension culture for enhancing callus growth when N6 medium supplemented with 4.5 μM 2,4-D was used.
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