Purification and Evaluation of Rice Bran Hydrolysates with Antimutagenicity
2007
Heo, S. (Konkuk University, Seoul, Republic of Korea) | Hettiarachy, Navam (University of Arkansas, Fayetteville, AR, USA) | Park, J.S. (Dongguk University, Seoul, Republic of Korea) | Kim, H.I. (Dongguk University, Seoul, Republic of Korea) | Paik, H.D. (Konkuk University, Seoul, Republic of Korea) | Yun, M.S. (Konkuk University, Seoul, Republic of Korea) | Lee, S.K. (Konkuk University, Seoul, Republic of Korea), E-mail: lesikyung@konkuk.ac.kr
A 3% suspension of heat-stabilized defatted rice bran was treated with papain, followed by inactivating the enzyme by heat, and centrifuged. The supernatant was subjected to ultrafiltration, and fractions with various molecular sizes, F1 (greater than 30 kDa), F2 (10-30 kDa), F3 (5-10 kDa), F4 (3-5 kDa), and F5 (3 kDa less than), were freeze-dried, and evaluated for antimutagenicity by Ames test using Salmonella typhimurium TA 100 against phenazine methosulfate. The F3 fraction containing highest antimutagenicity from ultrafiltration was separated into 6 fractions by DEAE-Sephadex A-25 ion-exchange column chromatography (F3-1-F3-6).
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