pVC, a Small Cryptic Plasmid from the Environmental Isolate of Vibrio cholerae MP-1
2007
Zhang, Ruifu (The University of Hong Kong, Hong Kong SAR, P. R. China) | Wang, Yanling (The University of Hong Kong, Hong Kong SAR, P. R. China) | Leung, Pak Chow (The University of Hong Kong, Hong Kong SAR, P. R. China) | Gu, Ji-Dong (The University of Hong Kong, Hong Kong SAR, P. R. China), E-mail: jdgu@hkucc.hku.hk
A marine bacterium was isolated from Mai Po Nature Reserve of Hong Kong and identified as Vibrio cholerae MP-1. It contains a small plasmid designated as pVC of 3.8 kb. Four open reading frames (ORFs) are identified on the plasmid, but none of them shows homology to any known protein. Database search indicated that a 440 bp fragment is 96% identical to a fragment found in a small plasmid of another V. cholerae. Further experiments demonstrated that a 2.3 kb EcoRI fragment containing the complete ORF1, partial ORF4 and their intergenic region could self-replicate.
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