Determination of the Optimal Concentration of Fetal Bovine Serum for the Growth of Two Insect Cell and Viruses
2007
Lee, J.K. (Chungbuk National University, Cheongju, Republic of Korea) | Koo, H.N. (Chungbuk National University, Cheongju, Republic of Korea) | Woo, S.D. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: [email protected]
To determine the optimal concentration of fetal bovine serum (FBS) on the growth of insect cells and the multiplicity of viruses, the growth of cells (Sf21 and Bm5) and viruses were examined on the various concentrations of FBS. In view of the viability, growth speed, proliferation of cells and the amount of FBS, the most proper concentration for the cell culture were 7% and 5% for Sf21 and Bm5, respectively. The multiplicity of viruses at the various concentrations of FBS was similar in both cell lines at 5 days post-infection (p.i.).
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