Application of Sodium Propionate to the Suspension Culture of Chinese Hamster Ovary Cells for Enhanced Production of Follicle-Stimulating Hormone
2007
Yoon, S.K. (LG Life Sciences, Ltd., Daejeon, Republic of Korea), E-mail: skyoonb@lgls.co.kr | Ahn, Y.H. (LG Life Sciences, Ltd., Daejeon, Republic of Korea)
In this study, we found that adding sodium propionate to batch culture medium resulted in cell growth suppression in a dose-dependent manner, while it enhanced follicle-stimulating hormone (FSH) production in Chinese hamster ovary (CHO) cells. In a pseudo-perfusion culture, with the exchange of fresh medium containing 2.5 mM sodium propionate, a final FSH of 532 ㎍ was obtained, which was approximately 1.5-fold higher than the control. Overall, the results demonstrate that the application of sodium propionate for the commercial production of recombinant proteins in rCHO cells is feasible.
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