Extracellular Secretion of a Maltogenic Amylase from Lactobacillus gasseri ATCC33323 in Lactococcus lactis MG1363 and its Application on the Production of Branched Maltooligosaccharides
2007
Cho, M.H. (Kyung Hee University, Yongin, Republic of Korea) | Park, S.E. (Kyung Hee University, Yongin, Republic of Korea) | Lee, M.H. (Kyung Hee University, Yongin, Republic of Korea) | Ha, S.J. (Kyung Hee University, Yongin, Republic of Korea) | Kim, H.Y. (Kyung Hee University, Yongin, Republic of Korea) | Kim, M.J. (University of Inje, Gimhae, Republic of Korea) | Lee, S.J. (Korea University, Seoul, Republic of Korea) | Madsen, Soren M. (Bioneer A/S, Horsholm, Denmark) | Park, C.S. (Kyung Hee University, Yongin, Republic of Korea), E-mail: cspark@khu.ac.kr
A maltogenic amylase gene from Lactobacillus gasseri ATCC33323 (LGMA) was expressed in Lactococcus lactis MG1363 using the P170 expression system. The successful production of recombinant LGMA (rLGMA) was confirmed by the catalytic activity of the enzyme in liquid and solid media. The N-terminal amino acid sequencing analysis of the rLGMA showed that it was Met-Gln-Leu-Ala-Ala-Leu-, which was the same as that of genuine protein, meaning the signal peptide was efficiently cleaved during secretion to the extracellular milieu.
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