Cloning and Characterization of a PI-like MADS-Box Gene in Phaladenopsis Orchid
2007
Guo, Bin (Fudan University, Shanghai, China) | Hexige, Saiyin (Fudan University, Shanghai, China) | Zhang, Tian (Fudan University, Shanghai, China) | Pittman, Jon K. (University of Manchester, Manchester, UK) | Chen, Donghong (Fudan University, Shanghai, China) | Ming, Feng (Fudan University, Shanghai, China), E-mail: fming@fudan.edu.cn
The highly evolved flowers of orchids have colorful sepals and fused columns that offer an opportunity to discover new genes involved in floral development in monocotyledon species. In this investigation, we cloned and characterized the homologous PISTALLATA-like (PI-like) gene PhPI15 (Phalaenopsis PI STILLATA # 15), from the Phalaenopsis hybrid cultivar. The protein sequence encoded by PhPI15 contains a typical PI-motif. Its sequence also formed a subclade with other monocot PI-type genes in phylogenetic analysis.
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