Inhibition of Gap Junctional Intercellular Communication in Rat Liver Epithelial Cells Induced by BHT and Propyl Gallate
2007
Kim, J.S. (Korea Food Research Institute, Seongnam, Republic of Korea) | Kim, S.R. (Korea Food Research Institute, Seongnam, Republic of Korea) | Ahn, J.Y. (Korea Food Research Institute, Seongnam, Republic of Korea) | Ha, T.Y. (Korea Food Research Institute, Seongnam, Republic of Korea) | Kang, K.S. (Seoul National University, Seoul, Republic of Korea) | Kim, S.A. (Korea Food Research Institute, Seongnam, Republic of Korea), E-mail: suna@kfri.re.kr
This study was conducted to analyze the cytotoxic effects of butylated hydroxytoluene (BHT) and propyl gallate (PG) in WB-F344 rat liver epithelial cells. Here we measured the inhibition level of gap junctional intercellular communication (GJIC) and elucidated the relationships between GJIC and mitogen-activated protein kinases (MAPKs) such as ERK, JNK, and p38. The cytotoxicities of BHT and PG appeared at concentrations of 1.0 mM and 0.25 mM, respectively, in the WB-F344 cells; and GJIC inhibition, which was analyzed by a scrape-loading/dye transfer assay and Western blotting analysis, appeared at 0.6 mM for BHT and 0.1 mM for PG, respectively.
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