Influence of donor plant growth condition, microspore isolation method, culture medium, and light culture on the production of embryos in microspore culture of hot pepper (Capsicum annuum L.)
2007
Lee, J.S. (Mokwon University, Daejeon, Republic of Korea) | Park, E.J. (Mokwon University, Daejeon, Republic of Korea) | Kim, M.Z. (Mokwon University, Daejeon, Republic of Korea), E-mail: kim70@mokwon.ac.kr
To establish an efficient and reliable microspore culture system for pepper (Capsicum annuum L.), the effect of light intensity used for donor plant's growth, microspore isolation methods, the composition of culture medium, and culture period in light on the production of embryos were investigated. The viability of microspores taken from the plants grown under the light intensity of 10,000 lux was almost same as that from the lower (5,500 lux) light intensity, and the embryo induction and development were a bit higher when donor plants were grown under the lower light intensity. This result implies that lower light intensity does not interfere with the embryo induction and development. However, it was very difficult to prepare microspores for culture since only a small number of flower buds could be harvested from plants grown under the light intensity of 5,500 lux.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Korea Agricultural Science Digital Library
