Typing of Extended-Spectrum β-Lactamase of Escherichia coli and Klebsiella pneumoniae Isolated from Rivers in Busan, Korea
2007
Lee, H.K. (Pukyong National University, Busan, Republic of Korea), E-mail: hunku@pknu.ac.kr | Kim, H.J. (Pukyong National University, Busan, Republic of Korea) | Kim, G.D. (Pukyong National University, Busan, Republic of Korea)
The purpose of this study was typing the plasmid mediated extended spectrum β-lactamases produced by enteric bacteria isolated from rivers in Pusan. Six strains of Eschericha coli and fifteen strains of Klebsiella pneumoniae transferred their plasmid mediated extended spectrum β-lactamase genes to the recipient strain Eschericha coli J53 Azid∨R. The plasmid mediated extended spectrum β-lactamase genes were sequenced directly after PCR and the types were determined by the BCM Search Launcher and GenBank nucleotid database. Determined types of the plasmid mediated extended spectrum β-lactamases were TEM-52 and SHV-12. TEM-52 was isolated from both Escherichia coli and Klebsiella pneumoniae. However SHV-12 was isolated from Klebsiella pneumoniae only. The results indicated that the plasmid mediated extended spectrum β-lactamase producing bacteria spreded over the area of clinical to the nature in Korea.
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