Effect of Repeated Administration of Silk Peptide on the Immune System of Rats
2008
Ryu, J.M. (PeerBiotech Co., Ltd., Cheongju, Republic of Korea) | Kim, T.M. (PeerBiotech Co., Ltd., Cheongju, Republic of Korea) | Seo, I.K. (PeerBiotech Co., Ltd., Cheongju, Republic of Korea) | Yeon, S.H. (Worldway Co., Ltd., Jeoneui, Republic of Korea) | Lim, W.T. (Worldway Co., Ltd., Jeoneui, Republic of Korea) | Lee, J.Y. (Worldway Co., Ltd., Jeoneui, Republic of Korea) | Hwang, S.Y. (Juseong College, Cheongwon, Republic of Korea) | O, N.G. (Chungbuk National University, Cheongju, Republic of Korea) | Song, J.G. (Chungbuk National University, Cheongju, Republic of Korea) | Lee, J.S. (Chungbuk National University, Cheongju, Republic of Korea) | Kim, Y.B. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: solar93@cbu.ac.kr
In order to elucidate the possibility of influence on the immune system, silk peptide was administered to rats for 4 weeks. Male and female Sprague-Dawley rats were orally treated with low (500 mg/kg) or high (2,000 mg/kg) doses of silk peptide, and complete blood counts, organ weights, histopathology and cellularity of immune systems were examined. Silk peptide did not cause clinical signs, although the body weight gain at the high dose was slightly reduced. In hematological analysis, the number of white blood cells (WBC) and differential counts, the number of red blood cells (RBC) and RBC indices, and the number of platelets and mean platelet volume (MPV) were not affected by the administration of silk peptide. Especially, there were no differences in the number and rate (%) of immune cells such as lymphocytes as well as monocytes and granulocytes between vehicle and silk peptide-treated groups. Silk peptide neither affected the thymus and spleen weights, in addition to the cellularity in thymus, nor induced gross and microscopic lesions of thymus, spleen and lymph node. Taken together, 4-week administration of silk peptide up to 2,000 mg/kg, the upper-limit dose in repeated-dose toxicity study, did not exhibit adverse effects on the immune systems, leading to no observed adverse effect level (NOAEL) higher than 2,000 mg/kg. Without influence on the production, maturation and death process of immune cells at 20-fold dose of clinical dose (100 mg/kg), silk peptide was evaluated to be a practically non-toxic material.
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