Composition and Cytotoxicity of Essential Oil from Korean rhododendron (Rhododendon mucronulatum Turcz. var. ciliatum Nakai)
2008
Park, Y.H. (Kangwon National University, Chuncheon, Republic of Korea) | Kim, S.M. (Kangwon National University, Chuncheon, Republic of Korea), E-mail: [email protected]
The essential oil was obtained from the aerial part of Rhododendon mucronulatum Turcz. var. ciliatum Nakai by steam distillation, samples were collected by headspace (HS) and solid-phase microextraction (SPME) methods, and the compositions of the oil were analyzed by gas chromatography-mass spectrometry (GC-MS). Nineteen constituents were identified from the essential oil: 15 carbohydrates, 3 alcohols, and 1 acetates. Major constituents were 2-β-pinene (16.1%), camphene (11.9%), δ-3-carene (11.4%), d,l-limonene (9.5%), and γ-terpinene (9.5%). By SPME extraction, seventeen constituents were identified: 13 hydrocarbons, 1 alcohol, 1 nitrogen-containing compound, 1 acetate, and 1 amine. Major constituents of the SPME-extracted sample were cam phene (19.6%), 2-β-pinene (18.0%), δ-3-carene (17.4%), trimethyl hydrazine (9.7%), γ-terpinene (8.5%), and d,l-limonene (5.5%). By HS extraction, thirteen constituents were identified: 11 hydrocarbons, 1 alcohol, and 1 nitrogen-containing compound. Major constituents of the HS-extracted sample were camphene (25.8%), δ-3-carene (24.8%), 2-β-pinene (20.2%), d,l-limonene (5.4%), tricyclene (5.1%) and trimethyl hydrazine (4.6%). The fragrance of the essential oil was coniferous, balsamic, and woody, and the IC∧50 value of the essential oil was 0.030 ㎍/mg in MTT assay using UaCaT keratinocyte cell line.
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