A Simple and Rapid Method for Screening of Nocardioides simplex Mutant with High Cholesterol Oxidase Activity
2008
Jang, Y.S. (University of Suwon, Hwaseong, Republic of Korea) | Jeong, J.M. (University of Suwon, Hwaseong, Republic of Korea), E-mail: [email protected]
The present report describes a paper disc colorimetirc method for the measurement of CHO activity. This method is applicable to the screening of mutant colonies with high CHO productivity on agar plates. The major advantages of this method include its reliability and simple, rapid screening. Using this screening method, several high CHO-producing mutant strains were obtained from a random EMS mutagenesis, and quantitative assays were then performed for the actual measurement of their activities. These results revealed that CHO activity of the selected mutants was higher than that of the wild type; thus, the paper disc colorimetric method for the screening of CHO can be useful for a simple, rapid screening of mutant colonies on agar plates.
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