Control of MPF Activity and Nuclear Remodeling of Somatic Cell Nuclear Transfer Bovine Embryos by Chemical Treatments
2008
Choi, Y.L. (Kangwon National University, Chuncheon, Republic of Korea) | Lee, Y.M. (Kangwon National University, Chuncheon, Republic of Korea) | Kim, H.J. (Kangwon National University, Chuncheon, Republic of Korea) | Park, J.H. (Kangwon National University, Chuncheon, Republic of Korea) | Kwon, D.J. (Kangwon National University, Chuncheon, Republic of Korea) | Park, C.K. (Kangwon National University, Chuncheon, Republic of Korea) | Yang, B.K. (Kangwon National University, Chuncheon, Republic of Korea) | Cheong, H.T. (Kangwon National University, Chuncheon, Republic of Korea), E-mail: [email protected]
We attempted to control the maturation promoting factors (MPF) activity and nuclear remodeling of somatic cell nuclear transfer (NT) bovine embryos. Bovine ear skin fibroblasts were fused to enucleated oocytes treated with either 5 mM caffeine for 2.5 h or 0.5 mM vanadate for 0.5 h and activated. The nuclear remodeling type of the reconstituted embryos was evaluated 1.5 h after activation. MPF activity was assessed in enucleated and chemical treated oocytes before the injection of a donor cell. Effect of chemicals on the embryonic development was evaluated with parthenogenetic embryos. MPF activity increased significantly by caffeine treatment, but decreased by vanadate treatment (p less than 0.05). Caffeine or vanadate had no deleterious effect on the parthenogenetic embryo development. In caffeine treated group, premature chromosome condensation (PCC) was occurred in 72.2% of NT embryos (p less than 0.05). In contrast, vanadate induced the formation of a pronucleus-like structure (PN) in a high frequency (68.9%, p less than 0.05) without PCC (NPCC). Blastocyst development of NT embryos increased by treating with caffeine (30.3%), whereas decreased by treating with vanadate (11.4%) compared to control (22.1%, p less than 0.05). The results indicate that caffeine or vanadate can control of MPF activity and remodeling type of NT embryos, resulting in the increased or decreased in vitro development.
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