Optimization of Conditions for Isolating and Cultivating Bacillus sp. SE-103 with a Mesophilic Feather-Degrading Activity
2009
Chang, H.S., Sangji University, Wonju, Republic of Korea | Choi, I., Sangji University, Wonju, Republic of Korea
This study was carried out to investigate the possibility to utilize feather meal by bacterial strains. A bacterial strain SE-103 producing keratinolytic enzyme was isolated from the soil of the poultry slaughterhouses. It was identified as Bacillus sp. by judging from its morphological and physiological characteristics. Subsequently the optimal culture conditions for the production of keratinolytic protease by Bacillus sp. SE-103 were investigated. The composition of optimal medium was 3.0% glucose, 0.4% urea, 0.2% NaNO₃, and 0.15% KCl. In addition, optimal initial pH and temperature were 6.0 and 35℃, respectively.
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