Antiproliferative Effect of Extracts, Fractions and Compound from Vitex rotundifolia on Human Cancer Cells
2009
Kim, Y.A., Korea Maritime University, Busan, Republic of Korea | Lee, J.I., Korea Maritime University, Busan, Republic of Korea | Kim, H.J., Korea Maritime University, Busan, Republic of Korea | Kong, C.S., Pukyong National University, Busan, Republic of Korea | Nam, T.J., Pukyong National University, Busan, Republic of Korea | Seo, Y.W., Korea Maritime University, Busan, Republic of Korea
Whole plants of Vitex rotundifolia were extracted for 2 days with methylene chloride (CH₂Cl₂) followed by extraction of the residue for an additional 2 days. The same procedure was also applied using methanol (MeOH). The two crude extracts were combined and partitioned between CH₂Cl₂ and H₂O. The organic layer was further partitioned between n-hexane and 85% aq. MeOH, and the aqueous layer was also further fractionated with n-BuOH and H₂O, successively. From the 85% aq. MeOH fraction, one compound was isolated through the repeated HPLC. According to the results of physicochemical data including NMR and MS, the chemical structure of the compound was determined as artemetin (1). The antiproliferative effects of the crude extracts, fractions, and compound against HT1080, AGS, MCF-7 and HT-29 human cancer cells were compared with the control by using MTT assay. In the comparative analysis, the 85% aq. MeOH fraction exhibited the strongest antiproliferative effects on human cancer cell lines in a dose-dependent manner (p less than 0.05). In addition, exposure of compound 1 isolated from 85% aq. MeOH fraction led to strong antiproliferative effect in HT1080 cancer cell lines. These results suggest that the extracts and compound isolated from V. rotundifolia may be used as potential chemopreventive and chemotherapeutic agents.
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