Effects of dietary supplementation of high-dose folic acid on biomarkers of methylating reaction in vitamin B∧12-deficient rats
2009
Min, H.S., Hannam University, Daejeon, Republic of Korea
Folate is generally considered as a safe water-soluble vitamin for supplementation. However, we do not have enough information to confirm the potential effects and safety of folate Supplementation and the interaction with vitamin B∧12 deficiency. It has been hypothesized that a greater methyl group supply could lead to compensation for vitamin B∧12 deficiency. On this basis, the present study was conducted to examine the effects of high-dose folic acid (FA) supplementation on biomarkers involved in the methionine cycle in vitamin B∧12-deficient rats. Sprague-Dawley rats were fed diets containing either 0 or 100 ㎍ (daily dietary requirement) vitamin B∧12/kg diet with either 2 mg (daily dietary requirement) or 100 mg FA/kg diet for six weeks. Vitamin B∧12-deficiency resulted in increased plasma homocysteine (p less than 0.01), which was normalized by dietary supplementation of high-dose FA (p less than 0.01). However, FA supplementation and vitamin B∧12 deficiency did not alter hepatic and brain S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) concentrations and hepatic DNA methylation. These results indicated that supplementation of high-dose FA improved homocysteinemia in vitamin B∧12-deficiency but did not change SAM and SAH, the main biomarkers of methylating reaction.
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