Anti-inflammatory Effect and HPLC Analysis of Extract from Edible Cirsium setidens
2009
Lee, S.H., Kangwon National University, Chuncheon, Republic of Korea | Jung, M.J., Kangwon National University, Chuncheon, Republic of Korea | Heo, S.I., Kangwon National University, Chuncheon, Republic of Korea | Wang, M.H., Kangwon National University, Chuncheon, Republic of Korea
The anti-inflammatory effect of Cirsium setidens (C. setidens) roots was evaluated for its potential to inhibit nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW 264.7 cells. The ethanol (EtOH) extract of C. setidens exhibited strong anti-inflammatory activities in the NO production by LPS-stimulated RAW 264.7 cells. The individual fractions tested were, in order of most-to-least potent in anti-inflammatory activity: n-butanol (n-BuOH) greater than ethanol (EtOH) greater than Water (H₂O) greater than ethyl acetate (EtOAc) greater than dichloromethane (CH₂Cl₂). The n-BuOH soluble fraction, which exhibited the strongest anti-inflammatory activity, was further purified by repeated MCI gel, silicagel, and RP-18 gel column chromatography. Syringin, isolated from C. setidens roots for the first time, were found to inhibit NO production in LPS-induced RAW 264.7 cells. High performance liquid chromatography (HPLC) was used for the analysis of the syringin in the EtOH extract of Cirsumn species.
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