Effect of Bacteria Eliminated Sperm by Percoll Method on Sperm Quality and Embryo Cleavage in Miniature Pig
2009
Yoo, H.J. (Kangwon National University, Chuncheon, Republic of Korea) | Jeon, J.M. (Kangwon National University, Chuncheon, Republic of Korea) | Lee, Y.S. (Kangwon National University, Chuncheon, Republic of Korea) | Cheong, H.T. (Kangwon National University, Chuncheon, Republic of Korea) | Yang, B.K. (Kangwon National University, Chuncheon, Republic of Korea) | Kim, D.Y. (Gachon University of Medical and Science, Incheon, Republic of Korea) | Park, C.K. (Kangwon National University, Chuncheon, Republic of Korea), E-mail: parkck@kangwon.ac.kr
The objectives of this study was to evaluate the efficiency of the bacteria eliminated sperm by percoll gradient method on sperm quality and embryo cleavage in vitro in pig. The semen of miniature pig collected by gloved-hand method pre-warmed (37℃) in thermos bottle, and separated by 65% percoll. Analysis of sperm ability was estimated by examining viability, capacitation and acrosome reaction using chlortetracycline (CTC) and the abnormality. Also, fertility of sperm was monitored with cleavage rate of embryo after IVF using separated and un-separated sperm by percoll. The result, viability of separated sperm was significantly(p less than 0.05) higher(83.6±2.0 vs 59.0±4.4%) than un-separated sperm. The results of CTC analysis showed the percentage of F- and B-patterned separated sperm was higher in separated that than un-separated sperm. On the contrary, the percentage of AR-patterned form unseparaed sperm was significantly(p less than 0.05) higher(13.6±0.8 vs 8.1±0.6%) than separated sperm. Also, abnormality of un-separated sperm was significantly(p less than 0.05) higher(20.2±0.4 vs 16.8±2.8%) than separated sperm. However, the cleavage rates of embryo using separated sperm by percoll and un-separated sperm had not significantly difference on 2 cell stage(9.25 vs 11.88%), 4 cell stage(26.76 vs 24.51%) and greater than 4 cell stage(63.99 vs 63.61%) at 48h of IVF. Therefore, the sperm separated by percoll method showed improvement in sperm quality than un-separated sperm in miniature pig.
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