Antioxidant Activity of the Salt Marsh Plant Corydalis heterocarpa
2009
Kim, Y.A., Korea Maritime University, Busan, Republic of Korea | Lee, J.I., Korea Maritime University, Busan, Republic of Korea | Lee, J.H., Korea Maritime University, Busan, Republic of Korea | Kong, C.S., Pukyong National University, Busan, Republic of Korea | Nam, T.J., Pukyong National University, Busan, Republic of Korea | Seo, Y.W., Korea Maritime University, Busan, Republic of Korea
The antioxidant activities of two crude extracts (CH₂Cl₂ and MeOH) and their solvent fractions (n-hexane, 85% aq. MeOH, n-BuOH, and H₂O fractions) from Corydalis heterocarpa were determined by evaluating authentic ONOO- and ONOO- generated from SIN-1 (3-morpholinsydnonimine) in vitro as well as by measuring the degree of occurrence of intracellular reactive oxygen species (ROS) and nitric oxide (NO). Scavenging activities of solvent fractions on authentic ONOO- increased in the order of n-BuOH greater than 85% aq. MeOH greater than H₂O greater than n-hexane fractions, while those on ONOO- generated from SIN-1 increased in the order of n-BuOH greater than 85% aq. MeOH greater than H₂O greater than n-hexane fractions. In addition, all solvent fractions effectively inhibited the intracellular ROS and NO levels. The n-BuOH fraction especially exhibited the strongest ROS scavenging effect. Further purification of n-BuOH fraction led to the isolation of cnidimoside A, which presented the potent ROS scavenging effect at 10 μM. From these results, extracts of C. heterocarpa and its component, cnidimoside A, were predicted to be potentially useful as ingredients for protecting against oxidation.
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