Effect of Culture Condition on Hanwoo Embryonic Developments and Their Survival after Vitrification
2010
Cho, S.R., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Choi, S.H., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Choe, C.Y., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Son, J.K., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Lee, P.Y., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Ko, Y.K., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Kim, H.J., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Yeon, S.H., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Son, D.S., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea
We investigated the cleavage rate and blastocyst yield for each culture condition to enhance tolerance of cryopreservation of bovine IVF embryo with relatively lower cryo-tolerance compared to in vivo embryo. The cleavage rate and blastocysts yield for CR1aa, IVMD, IVD, CR1aa+10% FBS were 73.2, 69.3, 72.8, 68.5% and 44.1, 30.8, 33.3, 48.0%, respectively. The values did not differ among each treatments without serum. For embryo vitrification, In vivo and In vitro blastocysts were exposed to VS1 (10% glycerin, 0.1 M glucose, 0.1 M sucrose, PEG 1%) for 5 min, and VS2 (10% glycerin, 10% EG, 0.2 M glucose, 0.2 M sucrose, PEG 2%) for 5 min and then VS3 (10% glycerin, 30% EG, 0.3 M glucose, 0.3 M sucrose, PEG 3%) for 1 min. The exposed embryos were then loaded into the 0.25 ml plastic straws and then plunged into liquid nitrogen. The straws were held for period of 1 to 2 weeks before thawing. In embryo viability, no differences in blastocyst re-expansion rates were found between in vivo and in vitro embryos. whereas expansion-BL rates was significantly higher for in vivo-derived embryos (72.7%) when compared to in vitro-derived embryos (51.4%), respectively (P less than 0.05). In conclusion, our results indicate that combined use of CRIaa culture medium with vitrification might enhance tolerance of cryopreservation for bovine IVF embryo production.
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