TLE-1 mRNA Expression during In Vivo and In Vitro Maturation in Porcine Oocytes
2011
Jang, Y.J., Kangwon National University, Chuncheon, Republic of Korea | Kim, D.W., Kangwon National University, Chuncheon, Republic of Korea | Lee, Y.S., Kangwon National University, Chuncheon, Republic of Korea | Cheong, H.T., Kangwon National University, Chuncheon, Republic of Korea | Yang, B.K., Kangwon National University, Chuncheon, Republic of Korea | Park, C.K., Kangwon National University, Chuncheon, Republic of Korea
Transducin-like enhancer protein 1(TLE-1) is protein associated with cell proliferation. This study analyzed change of TLE-1 mRNA expression during in vivo and in vitro maturation in porcine oocytes. Oocytes and granulose cells were collected from follicles of less than 2 mm, 2~6 mm and greater than 6 mm in diameter in slaughtered pig's ovaries. Oocytes collected from follicles of 2~6 mm in diameter were used after in vitro maturation for 0, 10, 20 and 44 h. Cumulus cells and granulose cells were collected after treatment with hyaluronidase. In results, TLE-1 mRNA expression in oocytes collected from follicle greater than 6 mm in diameter is increased, TLE-1 RNA expression in cumulus cells and granulosa cells from follicles less than 2 mm, 2 mm 6 mm and greater than 6 mm in diameter. However, there is no significant difference. On the other hand, TLE-1 mRNA expression from oocytes cultured for 10 hand 44 h is increased, TLE-1 mRNA in cumulus cells cultured for 10 h is significant increased(p less than 0.05) than other culture periods. In conclusion, these results show that TLE-1 is expressed in all cell types of oocytes, cumulus cells and granulose cells, and associated with oocyte maturation.
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