Isolation, Identification, and Characterization of Ornithine-Producing Enterococcus faecalis OA18 from Kefir Grain
2011
Yu, J.J., Woosuk University Graduate School, Jeonju, Republic of Korea | Kim, S.G., Woosuk University Graduate School, Jeonju, Republic of Korea | Seo, K.W., Woosuk University Graduate School, Jeonju, Republic of Korea | Oh, S.H., Woosuk University Graduate School, Jeonju, Republic of Korea
Lactic acid bacteria (LAB) OA18 was isolated from yogurt prepared by using Kefir Grain as a starter. The OA18 strain was a Gram-positive, cocci-type bacterium, and able to grow anaerobically with CO₂ production. The OA18 strain grew well on MRS broth supplemented with 50 mM arginine at 30-37℃ and pH of 7.0-9.0. The optimum temperature and pH for growth are 37℃ and pH 7.0. The isolate fermented ribose, D-glucose, cellobiose, D-trehalose, but not L-xylose, D-melibiose, and inositol. The 16S rRNA gene sequence of the isolate showed 99.8% homology with the Enterococcus faecalis 16S rRNA gene (Access no. AB012212). Based on the biochemical characteristics and 16S rRNA gene sequence analysis data, it was identified and named as E. faecalis OA18. The E. faecalis OA18 strain showed a high ornithine-producing capacity in the presence of arginine and also showed an antimicrobial activity against Streptomyces strains such as Streptomyces coelicolor subsp. Flavus, S. coeruleorubidus, S. coeruleoaurantiacus, S. coelicolor, S. coeruleoprunus. The cell growth of E. faecalis OA18 strain was maintained in MRS broth with a NaCl concentration of 0-7%.
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