Paenibacillus xylaniclasticus sp. nov., a Xylanolytic-Cellulolytic Bacterium Isolated from Sludge in an Anaerobic Digester
2012
Tachaapaikoon, Chakrit, King Mongkut's University of Technology Thonburi, Bangkok, Thailand | Tanasupawat, Somboon, Chulalongkorn University, Bangkok, Thailand | Pason, Patthra, King Mongkut's University of Technology Thonburi, Bangkok, Thailand | Sornyotha, Somphit, King Mongkut's University of Technology Thonburi, Bangkok, Thailand | Waeonukul, Rattiya, King Mongkut's University of Technology Thonburi, Bangkok, Thailand | Kyu, Khin Lay, Chulalongkorn University, Bangkok, Thailand | Ratanakhanokchai, Khanok, Chulalongkorn University, Bangkok, Thailand
A mesophilic, facultative, anaerobic, xylanolytic-cellulolytic bacterium, TW1∨T, was isolated from sludge in an anaerobic digester fed with pineapple waste. Cells stained Gram-positive, were spore-forming, and had the morphology of straight to slightly curved rods. Growth was observed in the temperature range of 30 to 50℃ (optimum 37℃) and the pH range of 6.0 to 7.5 (optimum pH 7.0) under aerobic and anaerobic conditions. The strain contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The predominant isoprenoid quinone was menaquinone with seven isoprene units (MK-7). Anteiso-C∧15:0, iso-C∧16:0, anteiso-C∧17:0, and C∧16:0 were the predominant cellular fatty acids. The G+C content of the DNA was 49.5 mol%. A phylogenetic analysis based on 16S rRNA showed that strain TW1∨T belonged within the genus Paenibacillus and was closely related to Paenibacillus cellulosilyticus LMG 22232∨T, P. curdlanolyticus KCTC 3759∨T, and P. kobensis KCTC 3761∨T with 97.7, 97.5, and 97.3% sequence similarity, respectively. The DNA-DNA hybridization values between the isolate and type strains of P. cellulosilyticus LMG 22232∨T, P. curdlanolyticus KCTC 3759∨T, and P. kobensis KCTC 3761∨T were found to be 18.6, 18.3, and 18.0%, respectively. The protein and xylanase patterns of strain TW1∨T were quite different from those of the type strains of closely related Paenibacillus species. On the basis of DNA-DNA relatedness and phenotypic analyses, phylogenetic data and the enzymatic pattern presented in this study, strain TW1∨T should be classified as a novel species of the genus Paenibacillus, for which the name Paenibacillus xylaniclasticus sp. nov. is proposed. The type strain is TW1∨T (=NBRC 106381∨T =KCTC 13719∨T =TISTR 1914∨T).
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