Repellent Activity of the Extracts of Acorus Gramineus against Mosquito (Culex pipinens pallens)
2011
Lee, S.H., Chonnam National University, Gwangju, Republic of Korea | Jung, J.K., Youngil High School, Seoul, Republic of Korea | Park, S.H., Youngil High School, Seoul, Republic of Korea | Kim, M.S., Youngil High School, Seoul, Republic of Korea | Han, Y.S., Chonnam National University, Gwangju, Republic of Korea | Seo, J.M., Gwangju Advanced Institute of Health and Environment, Gwangju, Republic of Korea | Kim, I.S., Chonnam National University, Gwangju, Republic of Korea
Acorus gramineus is of medicinal plants that exhibit variable biological activities for human health and against insect pests. The extracts of A. gramineus was examined in an attempt to develop a natural repellent against human disease-mediating Culex pipiens. METHODS AND RESULTS: The roots of A. gramineus dried under dark conditions were homogenized and extracted with ethanol. The extracts were subjected to repellent activity assays against C. pipiens in a hand-made acrylamide box with three accessible rooms. Significantly low number of mosquitos was found in the room previously fumigated with the extracts at 50 mg/L on the filter paper, exhibiting less than 20% of mosquitos tested. More than 50% of mosquitos tested was found in the room without the extracts, but less than 30% was found in the room that released mosquitos. GC/MS analysis detected β-asarone as a main component of the extracts. The commercial asarones (α and β) showed a repellent activity at 50 mg/L on the filter paper similar to the extracts. CONCLUSION(S): A. gramineus has potential for use as a mosquito repellent since β-asarone, a main component of the plant, exhibited a strong repellent activity against C. pipiens.
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