Comparison of Virulence Factors of Enterococci from Intestinal Drugs, Infant Feces and Clinical Isolates
2013
Lee, J.H., Gyeongsang National University, Jinju, Republic of Korea | Hwang, S.W., Daewoo Pharmaceutical Company, Busan, Republic of Korea | Kang, K.R., Daewoo Pharmaceutical Company, Busan, Republic of Korea | Kim, D.H., Daewoo Pharmaceutical Company, Busan, Republic of Korea | Kim, C.G., Inje University, Inje, republic of Korea
Three isolates, E.faecium P1,P2 and P3, from intestinal drugs of three phamaceutical companies, four clinical vancomycin resistant isolates, E, faecium V1, V2, V3 and E, faecalis V4 , and three isolates, E. faecalis DW01, DW07 and DW14, from infant feces were tested for the presence of virulence genes, ace, agg, esp, efaA, gelE, sprE,vanA and vanB as well as fsrABC, regulatory genes of gelE and sprE, cylMB4, cytolysin activation genes and cpd, cob and ccf, pheromone genes by PCR and for their phenotype activities such as protease, biofilm formation, cell clumping and hemolysis. The genes encoding cell surface adherence proteins, ace, agg, esp and efaA, were predominantly amplified from the canceomycin resistant strain V4 and the fecal isolates DW01, DW07 and DW14. Both protease and biofim formation activity were detected only from E. faecalis V4 from which the PCR products of gelE and spreE as well as fsrABC were amplified. The pheromone genes were amplified from the V4, DW01, DW07 and DW14 strains and these strains showed clumping activity . Biofilm formation was observed from the strains DW01, DW07 and DW14, all of chich produced PCR products of pheromone, and V4 as well. Whole cytolysis regulator genes were amplified from DW01,DW07 and DW14 and b-hemolysis activity was detected from these strains. Any virulence genes or activities except the pheomone gene ccf were not detected from the pharmaceutical isolates, E. faecium P1, P2 and P3.
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