Comparison of Conventional Culture Method and Real-time PCR for Detection of Yersinia enterocolitica in Sausage and Vegetable Salad
2013
Kim, Y.K., Konkuk University, Republic of korea | Chon, J.W., Konkuk University, Republic of korea | Lee, J.H., Konkuk University, Republic of korea | Kwak, H.S., National Institute of Food and Drug Safety Evaluation, Republic of Korea | Hwang, I.G., National Institute of Food and Drug Safety Evaluation, Republic of korea | Seo, K.H., Konkuk University, Republic of korea
The purpose of this study was to compare a conventional culture method and real-time PCR for the detection of Yersinia enterocolitica (Y. enterocolitica) in sausage and in vegetable salad. Food samples inoculated with Y. enterocolitica were enriched in peptone-sorbitol bile-broth, and swabs were then streaked onto cefsulodin-irgasannovobiocin agar. Biochemical tests for suspected colonies were performed with an API 20E strip. In parallel, real-time PCR was performed, targeting the 16S rRNA gene using 1 mL of enrichment broth. In sausage, the number of positive samples detected by culture method (49 out of 60) was similar (p0.05) with that of real-time PCR (50 out of 60). However, the number of positive samples of real-time PCR (26 out of 60) was significantly higher (p0.05) than that of the conventional culture method (6 out of 60) in vegetable salad. Real-time PCR could be an effective screening tool for
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