Analysis and Monitoring of Aflatoxin M₁ in Milks
2012
Park, S.K., Korea Food and Drug Administration, Cheongwon, Republic of Korea | Kang, Y.W., Korea Food and Drug Administration, Cheongwon, Republic of Korea | Kwon, K.S., Korea Food and Drug Administration, Cheongwon, Republic of Korea | Lee, G.H., Korea Food and Drug Administration, Cheongwon, Republic of Korea | Kim, M.H., Korea Food and Drug Administration, Cheongwon, Republic of Korea
Raw milk samples (n=28) obtained from milk tanks in 3 dairy plants of different regions and commercial milks (n=100) were collected from six cities. These samples were analyzed for the level of aflatoxin M₁ contamination using immunoaffinity columns and high performance liquid chromatography coupled with fluorescent detectors. Confirmation of aflatoxin M₁ (AFMM₁) identified in positive samples was based on the formation of the hemiacetal derivative (AFM∧2a) after derivatization with trifluroacetic acid. The average concentrations of aflatoxin M₁ in the raw milks were 25.1 ng/kg, and those values in commercial milks were 29.8 ng/kg. The highest level of aflatoxin M₁ in milk was 72.7 ng/kg. These results showed that the contamination of aflatoxin M₁ in milks consumed in the Korea was quite low compared to the standard in Korea Food Code (aflatoxin M₁ 500 ng/kg).
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