NIRS Calibration Equation Development and Validation for Total Nitrogen Contents Field Analysis in Fresh Rice Leaves

This study was evaluated high end research grade Near Infrared Reflectance Spectrophotometer (NIRS) to field grade multiple Near Infrared Reflectance Spectrophotometer (NIRS) for rapid analysis at fresh rice leaf at sight with 238 samples of fresh rice leaf during year 2012, collected Jeollabuk-do for evaluate accuracy and precision between instruments. Firstly collected and build database high end research grade NIRS using with 400 nm ~ 2500 nm during from year 2003 to year 2009, seven years collected fresh rice leaf database then trim and fit to field grade NIRS with 1200 nm ~ 2400 nm then build and create calibration, transfer calibration with special transfer algorithm. The result between instruments was 0.005% differences, rapidly analysis for chemical constituents, Total nitrogen in fresh rice leaf within 5 minutes at sight and the result equivalent with laboratory data. Nevertheless last during more than 8 years collected samples for build calibration was organic samples that make differentiate by local or yearly bases etc. This strongly suggest population evaluation technique needed and constantly update calibration and maintenance calibration to proper handling database accumulation and spread out by knowledgable control laboratory analysis and reflect calibration update such as powerful control center needed for long lasting usage of fresh rice leaf analysis with NIRS at sight. Especially the agriculture products such as rice will continuously changes that made easily find out the changes and update routinely, if not near future NIRS was worthless due to those changes. Many research related NIRS was shortly study not long term study that made not well using NIRS, so the system needed check simple and instantly using with local language supported signal methods global distance (GD) and neighbour distance (ND) algorithm. Finally the multiple popular field grades instruments should be the same results not only between research grade instruments but also between multiple field grade instruments that needed easily transfer calibration and maintenance between instruments via internet networking techniques.