Genetic Analysis and Molecular Mapping of Low Amylose Gene in Rice Cultivar 'Baegjinju' (Oryza sativa L.)
2016
Lee, J.H., Research Policy Bureau , RDA, Jeonju, Republic of Korea | Cho, J.H., Paddy Crop Research Division, NICS, RDA, Miryang, Republic of Korea | Lee, J.Y., Paddy Crop Research Division, NICS, RDA, Miryang, Republic of Korea | Sohn, Y.B., Paddy Crop Research Division, NICS, RDA, Miryang, Republic of Korea | Han, S.I., Paddy Crop Research Division, NICS, RDA, Miryang, Republic of Korea | Shin, D., Paddy Crop Research Division, NICS, RDA, Miryang, Republic of Korea | Song, Y.C., Paddy Crop Research Division, NICS, RDA, Miryang, Republic of Korea | Park, D.S., Paddy Crop Research Division, NICS, RDA, Miryang, Republic of Korea | Yeo, U.S., Paddy Crop Research Division, NICS, RDA, Miryang, Republic of Korea | Oh, M.G., Paddy Crop Research Division, NICS, RDA, Miryang, Republic of Korea | Hur, Y.J., Paddy Crop Research Division, NICS, RDA, Miryang, Republic of Korea
The amylose contents of rice determine eating quality which is one of the major traits in rice breeding program. To identify the low-amylose gene of the japonica rice cultivar Baegjinju, genetic analysis was conducted using 200 F2 population derived from a cross between the japonica cultivars, Saeilmi and Baegjinju. Individual F2 plants were classified as wild type (translucent grain) and mutant type (dull grain) based on the grain appearance of brown rice. Two hundred F2 plants were segregated into 155 wild type plants and 45 mutant type plants, which fit the 3:1 ratio and this result indicated the low-amylose gene of Baegjinju is a single recessive gene which controls the amylose contents. Linkage analysis was conducted to localize the low-amylose gene of Baegjinju and fine mapped within an 800-kb interval between 17.5 to 18.8Mb on short arm of chromosome 10. Co-segregated SSR marker, RM25648 was developed and it could be useful for marker-assisted selection and determination of the genetic resource related with amylose contents in rice breeding.
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