The Effective Method to Screening High Iron Content Brown Rice
2016
Hwang, W.H., National Institute of Crop Science (NICS), RDA, Jeonju, Republic of Korea | Back, J.S., National Institute of Crop Science (NICS), RDA, Jeonju, Republic of Korea | An, S.H., National Institute of Crop Science (NICS), RDA, Jeonju, Republic of Korea | Jeong, J.H., National Institute of Crop Science (NICS), RDA, Jeonju, Republic of Korea | Jeong, H.Y., National Institute of Crop Science (NICS), RDA, Jeonju, Republic of Korea | Lee, H.S., National Institute of Crop Science (NICS), RDA, Jeonju, Republic of Korea | Yun, J.T., National Institute of Crop Science (NICS), RDA, Jeonju, Republic of Korea | Lee, G.H., National Institute of Crop Science (NICS), RDA, Jeonju, Republic of Korea | Choi, K.J., National Institute of Crop Science (NICS), RDA, Jeonju, Republic of Korea
We investigate the change of leaf chlorophyll content according to iron content in brown rice when cultivated on the iron limited solid MS medium. By cultivating wild-type and transgenic brown rice in a solid MS medium, we confirmed that iron deficiency chlorosis did not occur in MS media which were contained over 20% of iron content compared to normal MS condition. After selecting twenty kinds of Korean rice varieties, those brown rice were cultivated in solid MS media which were contained from 0 to 15% of iron content compared to normal MS condition then the leaf chlorophyll content was measured. The leaf chlorophyll content was changed according to iron content in brown rice when cultivated in solid MS medium which was contained 0 and 5% of iron content,. There was a strong correlation between iron content in brown rice and leaf chlorophyll content cultivated in solid MS medium with 5% of Fe content. Therefore we expect that analysis of leaf chlorophyll content after cultivated on MS medium with 5% iron content compared to normal MS media condition will be more simple and effective method to screening high iron content brown rice without measurement of iron content.
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