Molecular cloning and expression analysis of the highlyconserved eukaryotic translation initiation factor 5A (eIF-5A)fromAntheraea pernyi
2018
Dandan BIAN, Yancheng TeachersUniversity, Yancheng, People's Republic of China | Xiaoming ZHAO, Shanxi University, Shanxi, People's Republic of China | Li CHEN, Henan Institute of Sericultural Sciences, Henan, Republic of Korea | JiwuTIAN, Agriculture Department of Henan Province, Henan, Republic of Korea | Qiuning LIU, Yancheng TeachersUniversity, Yancheng, People's Republic of China | Chunlin ZHOU, Yancheng TeachersUniversity, Yancheng, People's Republic of China | Boping TANG, Yancheng TeachersUniversity, Yancheng, People's Republic of China
Eukaryotic initiation factor 5A (eIF-5A) is a highly conserved protein found in alleukaryotic organisms that plays a key role in the regulation of many cellularprocesses including translation elongation, cell proliferation, programmed cell death,mRNA turnover and decay, and abiotic stress responses. In this study, the eIF-5Agene from the Chinese oak silkwormAntheraea pernyi(Lepidoptera: Saturniidae)was characterized. The full-lengthApeIF-5AcDNA of 1056BPincludes a50-untranslated region (UTR) of 138BP,a30-UTR of 435BP, and an open readingframe of 483BPencoding a polypeptide of 160 amino acids. The deducedApeIF-5Aprotein shares 99 %, 82 %, and 72 % sequence identity with orthologs inBombyxmori,Drosophila melanogasterandHomo sapiens, indicating high conservationduring animal evolution. Real-time quantitative reverse transcription PCR revealedexpression in all four developmental stages and in all nine tissues tested, consistentwith an important role in development. After challenge with lipopolysaccharide,the expression levels ofApeIF-5Awere markedly upregulated. Phylogenetic analysisof amino acid sequences revealedA. pernyieIF-5A was closely related toB. morieIF-5A, consistent with traditional classification and other molecular data. The resultsindicate the potential value ofeIF-5Ain phylogenetic analysis.
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