Comparative In-silico Analysis of the Cold Responsive ICE1 and ICE2 Genes in Zoysiagrasses
2022
Kaur, C. | Kim, Y.S. | Youn, J.H. | Bae, E.J. | Lee, G.J.
Inducer of CBF (C-repeat binding factors) expression 1, 2 (ICE1/2) is an important transcription factor that plays a significant role in the cold stress response pathway. Zoysiagrasses are warm-season turfgrasses and are prone to browning at the starting of winter. By understanding the cold response mechanism, the cold tolerance of some zoysiagrasses such as Zoysia matrella or Z. pacifica showing less cold hardiness can be improved to withstand low temperatures. Arabidopsis and Z. japonica ICE1, 2 have already been characterized and reported. Using in-silico analysis, we predicted the putative ICE1, 2 genes in Z. matrella and Z. pacifica. The comparative sequence analyses revealed conservation of the bHLH (basic helixloop-helix) domain in the putative ICE1 and ICE2 genes in Z. matrella and Z. pacifica. Also, two copies of ICE2 were seen in Z. matrella. There were huge variations in the immediate regulatory region of the ICE1 and ICE2 genes which may be a reason for different cold tolerance levels in the three Zoysiagrasses. The predicted sequences of the ICE1,2 genes in Z. matrella and Z. pacifica can be utilized to further investigate interactions with CBF3 and their implications in cold stress tolerance, which will provide new insight into molecular mechanisms in regulating cold tolerance.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Korea Agricultural Science Digital Library