Isolation and Characterization of NAC Family Genes Responding to Environmental Stress in Creeping Bentgrass
2022
Kim, B.H. | Sun, H.J. | Lee, H.Y. | Kang, H.G.
All kinds of crops including foods, feeds, and turf grasses are damaged frequently by various environmental stresses such as drought, salt, and high temperature, which cause the loss of agronomic productivity and the decline in commercial quality. Many abiotic stress responsive genes have been identified in plants and they have been applied to breed crops tolerant to environmental stresses through a transgenic approach. In this study, by use of 4 combinations of degenerate primer PCR and Rsa I, a 4-cutter restriction enzyme recognizing GTAC, 9 independent cDNAs containing NAC domain sequence were isolated from creeping bentgrass (Agrostis stolonifera L. cv Penncross). Among them, the 5 clones including AsNAC18, AsNAC19, AsNAC20, AsNAC23, and AsNAC24 belonged to NAM and CUC2 subfamily regulating development and the other 4 including AsNAC16, AsNAC17, AsNAC21 and AsNAC22 belonged to ATAF and SNAC1 subfamily regulating defense to abiotic stress. In mRNA expression analysis, AsNAC16, AsNAC17 and AsNAC19 were upregulated in the senescence leaf. AsNAC17, AsNAC18, AsNAC22 and AsNAC24 were upregulated by dark stress and AsNAC22 mRNA was also increased by heat stress as well as dark. It is considered that these genes are important as a gene pool for plant biotechnology.
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