Reduction of Allergic Inflammation Water Extract of Saussurea involucrata (Kar. & Kir.) Sch. Bip. through Tyrosine-protein Kinase Lyn and Sky Tyrosine Phosphorylation
2021
Park, S.A. | Jang, Y.S. | Yang, Y.H.
BACKGROUND: Snow lotus [Saussurea involucrata (Kar. and Kir.) Sch. Bip.] is used in Chinese herbal medicine because of its antioxidant and anti-inflammatory effects. The mechanism of its anti-inflammatory effect on allergic response is unknown. METHODS AND RESULTS: We evaluated the effect of the aqueous extract of S. involucrata flowers on the allergic inflammatory response in dinitrophenyl-immunoglobulin E (DNP-IgE)-activated RBL-2H3 cells. Cell viability and β-hexosaminidase release from the activated RBL-2H3 cells were evaluated. The levels of proinflammatory molecules including tumor necrosis factor-alpha (TNF-α), interleukin (IL)-4, IL-10, IL-1β, and prostaglandin E2 in RBL-2H3 cells treated with 5% and 10% S. involucrata extract. Treatment with S. involucrata extract significantly suppressed the phosphorylation of protein kinase B (AKT), p38, c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK)1/2, and mitogen-activiated protein kinase (MEK) in a dose-dependent manner. S. involucrata extract also inhibited the Fc epsilon receptor 1 signaling pathway involving phosphorylation of Lyn, Syk, and phosphoinositide 3-kinase (PI3K), which is associated with the PI3K-AKT-ERK pathway and cytokine expression. A major mechanism of action of S. involucrata extract was to reduce the extent of the DNP-IgE-induced cytoskeletal change from globular to filamentous actin in RBL-2H3 cells. CONCLUSIONS: S. involucrata extract mediates its antiallergic effect by suppressing inflammation. The findings will be valuable in the development of S. involucrata extract as a phytomedicine with minimal side effects for allergic diseases.
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