Antisense RNA-mediated inhibition of GAMFAD2-1 encoding omega-6-desaturase
2009
Gupta, N.C. | Sinha, S.K. | Jolly, M. | Sachdev, A.
Antisense RNA-mediated inhibition of gene expression is a valuable tool to metabolically engineer the composition of oilseeds to improve their nutritional value and provide the functional properties required for various purposes. To improve the efficiency of fad 2-1 inhibition and to selectively suppress it in developing seeds of soybean an antisense construct was designed in two stages. In the first stage, the PCR amplified conserved fad 2-1 gene sequence was cloned in the antisense orientation with respect to seed-specific (vicilin) promoter derived from pCW66 vector. In the second stage, the chimeric cassette was inserted in a plant transforming vector carrying "bar" gene and kanamycin resistance gene for plant selection. Efficacy of the chimeric construct was checked in the model plant Arabidopsis thalia.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Indian Council of Agricultural Research
