Seminal plasma ascorbic acid level and its relationship to sperm characteristics in Murrah buffalo bulls
2013
MAURYA, S K | SINGH, O P | SRIVASTAVA, SUSHANT
unknown. Ascorbic acid levels were investigated in the seminal plasma of Murrah buffalo bulls and its relation was investigatedwith various parameters of sperm morphology. Semen samples were collected using bovine artificial vagina. Semenvolume, sperm concentration, initial motility, livability, sperm abnormality, hypo osmotic swelling, acrosomal integrityand post thaw motility were assessed using standard procedures. Ascorbic acid contents in the seminal plasma were estimated by reverse phase high performance liquid chromatography (RP-HPLC) procedure using UV detection at 254 nm. Bulls were divided into 3 categories depending on the extent of sperm abnormality. The mean ± SEM values ofseminal plasma ascorbic acid (mg/ dl) were 4.81 ± 0.08, 3.17 ± 0.09 and 1.79 ± 0.05 in good, medium and poor groupsof Murrah buffalo bulls. The seminal plasma ascorbic acid differed significantly in all 3 groups. The seminal plasmaascorbic acid level was positively correlated with spermatozoa with volume/ ejaculate (r2 = 0.710), conc./ ejaculate (r2= 0.915), initial motility (r2 = 0.944), livability (r2 = 0.933), acrosomal integrity (r2 = 0.955), HOS reactiveness (r2 = 0.946) and post-thaw motility (r2 = 0.906); whereas it was negatively correlated with sperm abnormality (r2 = –0.896).
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