Rapid detection of Fusarium oxysporum f. sp. ciceris from disease infested chickpea fields by loop-mediated isothermal amplification
2016
MAMTA SHARMA*, RAJU GHOSH, AVIJIT TARAFDAR and
unknown. Fusarium wilt (FW) in chickpea, caused by major fungal pathogen, Fusarium oxysporum f. sp. ciceris (Foc) results in10-40% economic losses worldwide. In field condition, several soil borne pathogens produce similar FW like symptoms in chickpea. Therefore, by visual symptoms, distinguish and diagnosis of FW disease is very difficult in field. Hence, there is real need of an accurate, rapid and inexpensive diagnosis method for FW. In this study, we developed hydroxynaphthol blue dye based a loop-mediated isothermal amplification (LAMP) assay targeting ef1á gene sequence for rapid diagnosis of FW in chickpea. The reaction was optimized using minimum 10 fg of Focg DNA at 63°C for 60 min. The characteristic sky blue colour and ladder like band in agarose gel of the final LAMP products indicated the positive reaction. This LAMP based tool will be very helpful to rapid diagnosis and monitoring the chickpea FW in the field.
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