Recombinant chicken anaemia virus viral protein 1 gene expression in insect cell system
2012
T, KARTHIKA | S, MANOHARAN | T, JAYALAKSHMI E | K, KUMANAN
unknown. CAV specific PCR positive tissue samples of Namakkal region available in the Department of Animal Biotechnology, Madras Veterinary College, were used in this study. A multiple host expression vector pTriEx 1.1 Neo was selected to clone the CAV VP1 gene (1353 bp) and transformed into BL21 (DE3) strain of E. coli and confirmed. The CAV VP1 recombinant pTriEx vector was co- transfected to Bacvector triple cut virus DNA with Sf9 insect cells. The plaques formed by CAV VP1 recombinant baculovirus were purified and propagated for protein expression. The recombinant CAV VP1 protein expression was confirmed by indirect FAT and SDS-PAGE analysis followed by western blot analysis using specific serum.
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