Inhibitory effects of nitric oxide on steroidogenesis by buffalo granulosa cells cultured in vitro
2013
SHANMUGAM, M | PANDITA, S | PALTA, P
unknown. This study examined the effects of S-nitroso-N-acetyl-penicillamine (SNAP), a nitric oxide (NO) donor and Nw-nitro-L-argininemethyl ester hydrochloride (L-NAME), a nitric oxide synthase inhibitor on estradiol-17β andprogesterone production by buffalo granulosa cells. Granulosa cells (3×105) from small (<5 mm diameter) or large (> 9mm diameter) follicles were cultured for 24 h under completely serum-free conditions in DMEM: nutrient mixture F-12Ham (1:1 ratio) supplemented with 10–7 M androstenedione, 5 mg/ml human apo-transferrin, 0.1% BSA, in the presenceor absence of FSH (8 ng/ml). Granulosa cells from large follicles produced higher estradiol-17β and progesterone thanthose from small follicles. SNAP reduced estradiol-17β and progesterone production at concentrations of 0.1 and 1 mMwhen used alone and in the presence of FSH by granulosa cells from follicles of both size categories. L-NAME (0.2, 1and 5 mM) had, however, no effect on estradiol-17β or progesterone production, alone or in the presence of FSH. Ourresults demonstrated strong inhibitory effects of NO on estradiol-17β and progesterone production by buffalo granulosacells from small and large follicles and indicated that it may be an autocrine/paracrine regulator of steroidogenesis bygranulosa cells in buffalo.
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