Evaluation of PCR method for diagnosis and identification of leishmania species in cutaneous lesions
2008
Keykhosravi. A,, Animal Group - Department of Biology – Teacher Training Univarsity of Sabzevar | Golbang, N., Animal Group - Department of biology – Faculty of Science – Isfahan University | Jahangirnejad, A., Microbiology Group - Department of Biology – Faculty of Science – Isfahan University
This study, was carried out in Isfahan an endemic region in Iran. A total of 108 samples of patients with cutaneous lesion were sampled for study that were suspected to cutaneous leishmaniasis. For comparison and study, sampling was carried out with to take of lesion exudates by filter paper for PCR. Small amount of material to take for microscopic smear examination. The PCR primers were designed to bind within the conserved regions of kDNA (kinetoplast DNA). In this study, two different amplified fragments for two species were identified from the kDNA. A band in 620 bp was identified for Leishmania major (MRHO/SU/59/P strain) and a band in 830 bp was identified for Leishmania tropica (MHOM/sudan/58/OD). Result of this study represented when samples be used for PCR with out cultured in medium, sensitivity of PCR and microscopic smear examination methods are equal. Infact, reason of some sample unconfirmed by PCR method is inhibitor presence. The PCR method is more precise to confirm If the samples cultured in medium and then PCR done. PCR can be used for differentiation and identification of Leishmania species in epidemiologic studies and vaccine product. Key words: kDNA, Cutaneous leishmaniasis, Microscope, Epidemiologic, Polymerase chain reaction (PCR)
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