The possibility of using ozone to reduce aflatoxin contamination of pistachio and aflatoxin producing fungi.
2008
Mir Abu Al-Fotuhi, Mansureh | Karami, Ruh Ol-Lah | Chit Saz, Mahdi | Tavakkoli, Maryam
One of the most important stage which suggesting to play an important role for aflatoxin and aflatoxin _ producing fungi contaminations of pistachio nuts is processing stages through pistachio terminals while the pistachio nuts are being contacted with the high population densities of fungi and fungal contaminations are initiated to pistachio nut surfaces. To study the role of chemical control for reducing the fungi and aflatoxin contaminations at terminal, ozone were used in this reseach. Previous studies have demonstrated that a novel source of ozone gas (O3) may be used to chemically degrade numerous mycotoxins, including aflatoxin. Subsequent in vitro analyses demonstrated detoxification of aflatoxin, suggesting a potential method to contrl aflatoxin and aflatoxin producing fungi. The objective of this study was to evaluate the capability of electrochemically produced ozone to degrade aflatoxin and controlling aflatoxin producing fungus in artificially contaminated pistachio nuts with an afatoxicogenic isolate of A. parasiticus. Ten certified non contaminated samples of pistachio nuts were inoculated with an aflatoxicogenic A. parasiticus isolate (MS542), and incubatet at 28 Cº for 15 days. Contaminated pistachio nuts were treated for 15 and 10 minutes with O3 at 10gr/hour in 1 kg batches. Treatments were included: positive control, 15 minutes ozone for dried pistachio nuts, 15 minutes for pistachio nuts in water, 10 minutes for dried pistachio nuts and negative control, and each treatment had two replicates. Ozone was electrochemically generated by an ozone generator was made by Jehad Technology and Engineering Institute. To study fungal contamination, diluted suspension of each sample plated on AFPA medium. Aflatoxin contaminations of the samples were estimated using reversed-phase high performance liquid chromatography (HPLC) + IAC. The linearity of the standard curve was proved (R2=0.9999) using 0.4-7.2 ng/ml standard samples of aflatoxin. Recovery of the method for 5 ng/g spiked samples for aflatoxins was 95 %. The results showed that A. parasiticus population density of ozone treated pistachio nuts was decreased 90- 92% and total aflatoxin contaminations of the treated pistachio nuts decreased 4۵- 74%.
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