Optimization of parameters for the transfer of foreign gene to cotton (Gossypium hirsutum L.) by particle bombardment
1999
Wasif, A.A. | Haris | Noor, S. | Hussain, T. | Riazuddin, S. (Punjab Univ., Lahore (Pakistan). National Center of Excellence in Molecular Biology)
Zygotic embryos of cotton variety MNH-93 (Gossypium hirsutum L.) were bombarded with tungsten particles coated with plasmid pActin1-D carrying a reporter gene encoding beta-glucuronidase (GUS). A helium pressure of 60 kg/cm, vacuum of 28 inch of Hg, target distance 22 cm, DNA and tungsten ratio 0.50 mew g: 240 mew g and one-time shot showed maximum transformation efficiency by expressing a high detectable beta-glucuronidase activity of GUS gene. This study will help in developing a protocol for the transformation of cotton varieties with agronomically important genes.
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